Phil Foden, Jack Grealish, Raheem Sterling and John Stones join Walker in the 27-man squad for the games against Italy, Germany and Hungary next month.
The right-back was left out by Gareth Southgate for the friendlies against Ivory Coast and Switzerland in March, but will have the opportunity to add to his 65 caps as the Three Lions look to qualify for next year’s Nations League finals.
Sterling will make his 75th appearance if involved in any of the four games, with England set to face Hungary twice during a 10 day period.
Southgate’s men face Marco Rossi’s side at the Puskas Arena on Saturday 4 June, before travelling to Germany three days later.
They then play Italy at Molineux on Saturday 11 June, with Wolves’ stadium also set to play host when the quartet of fixtures concludes against Hungary the following Tuesday, 14 June.
Goalkeepers: Jordan Pickford (Everton), Nick Pope (Burnley), Aaron Ramsdale (Arsenal)
Defenders: Trent Alexander-Arnold (Liverpool), Conor Coady (Wolverhampton Wanderers), Marc Guéhi (Crystal Palace), Reece James (Chelsea), James Justin (Leicester City) Harry Maguire (Manchester United), John Stones (Manchester City), Fikayo Tomori (AC Milan), Kieran Trippier (Newcastle United), Kyle Walker (Manchester City), Ben White (Arsenal).
Midfielders: Jude Bellingham (Borussia Dortmund), Conor Gallagher (Crystal Palace, loan from Chelsea), Mason Mount (Chelsea), Kalvin Phillips (Leeds United), Declan Rice (West Ham United), James Ward-Prowse (Southampton).
Forwards: Tammy Abraham (AS Roma), Jarrod Bowen (West Ham United), Phil Foden (Manchester City), Jack Grealish (Manchester City), Harry Kane (Tottenham Hotspur), Bukayo Saka (Arsenal), Raheem Sterling (Manchester City).